ABSTRACT

Objectives:

Patients on maintenance hemodialysis have a high mortality risk compared to the general population. There have been reported many risk factors that affect the mortality of these patients. The aim of this study was to evaluate the clinical and laboratory characteristics of these patients and to identify risk factors that contributed to their mortality.

Materials and Methods:

This study was a retrospective observational cohort study conducted between January 2018 and January 2020. At the start of the study, all of patients were clinically evaluated, and data regarding demographic, clinic, and laboratory characteristics were collected. At the end of 2-year follow-up period, the patients were separated into two groups according to their survival status. Data were analyzed between the survival and death groups. Logistic regression analysis was used to evaluate risk factors associated with mortality.

Results:

A total of 74 patients (53% females and 47% males) were included in the analysis. The mean age of the study population was 53.04±14.61 years. At the end of 2-year follow-up period, 21 (28.4) patients died, and four (5.4) received a kidney transplant. The leading cause of death was cardiovascular events (52.2%), followed by infections (43%). Compared with the survival group, the death group patients had higher rate of comorbid conditions, older age, required higher erythropoiesis stimulating agent doses, lower levels of serum creatinine and albumin, and higher levels of C-reactive protein (CRP). The presence of diabetes mellitus, cerebrovascular disease, and high CRP levels are independent risk factors for mortality.

Conclusion:

An early and careful evaluation is essential in the follow-up of the patients in order to improve survival in this group of patients. Large-scale multicenter studies are needed to better determine the causes of increased risk of mortality in maintenance hemodialysis patients.

Introduction

The mortality rate in maintenance hemodialysis (MHD) patients remains high, despite the technical and medical improvements in the last years (1,2). MHD patients have a high rate of mortality than that of the general population. Therefore, it is important to identify risk factors underlying mortality on MHD patients (3). Cardiovascular disease is very common in MHD patients and is the leading cause of death in these patients. However, non-cardiovascular causes of death are increased to the same extent as cardiovascular mortality on MHD patients (4,5).

The increased risks of cardiovascular morbidity and mortality in MHD patients is related to an increased prevalence of traditional cardiovascular risk factors, including advanced age, diabetes mellitus (DM), hypertension (HT), obesity, smoking, and dyslipidemia (6,7). In addition, the presence of multiple comorbid conditions is an increasingly common problem in MHD patients and is associated with worse cardiovascular outcomes (8). Systemic inflammation and malnutrition also play an important role, and are poor prognostic factors identified for incresed mortality (9,10). MHD patients using central venous catheter (CVC) have been associated with an enhanced inflammatory state (11). Markers of nutritional status, such as serum albumin, serum creatinine, body mass index (BMI), and serum lipids are related to mortality, as are inflammatory markers such as C-reactive protein (CRP) and interleukin-6 (12,13). Disorders of mineral metabolism have also been defined as risk factors contributing to mortality in hemodialysis patients, especially hyperphosphatemia, hyperparathyroidism, and an elevated calcium phosphorous product (14). Although the importance of non-cardivascular causes of death has been highlighted in MHD patients, it has been underestimated in clinical practice. In this context, the aim of the present study was to evaluate the risk factors associated with mortality in a cohort of patients on MHD.

Materials and Methods

Patients and Study Design

This was a retrospective observational cohort study conducted between January 2018 and January 2020 at the hemodialysis unit at Van Training and Research Hospital of University of Health Sciences in the city of Van, Turkey. We included 80 MHD patients in our study. Patients on hemodialysis therapy for less than 3 months, presence of autoimmune disease, malignancy, and acute or chronic infection, were excluded from the study. Six patients were excluded because of did not meet the criteria, two of them had hemodialysis therapy for less than 3 months, three had malignancies, and one had autoimmune disease.

At the start of the study, all of patients were clinically evaluated, and data regarding baseline demographic, clinic and laboratory parameters were obtained from the hospital database. Demographic and clinical parameters such as age, gender, BMI, smoking status, etiology of primary kidney disease, comorbidities, types of vascular access, hemodialysis frequency and duration, dialysis vintage, and medical prescriptions were collected. The total doses of erythropoietin used in the last year was recorded. In terms of common standardization, the doses of darbepoetin were indicated and per week of kilogram doses calculated. DM was defined based on the criteria of the American Diabetes Association or the use of medications such as oral hypoglycemic agents and/or insulin (15). HT was defined as a systolic blood pressure of at least 140 mmHg and a diastolic blood pressure of at least 90 mmHg and/or previously diagnosed HT and/or the use of antihypertensive medication (16). Patients with total serum cholesterol levels greater than or equal to 200 mg/dL or low-density lipoprotein (LDL) cholesterol above 100 mg/dL and triglycerides greater than 150 mg/dL or patients who were taking lipid-lowering drugs were considered dyslipidemia (17,18). Patients with a history of myocardial infarction, angioplasty or myocardial revascularization were considered to have coronary artery disease (CAD). Those patients with a history of stroke or transient ischemic attack or those who had undergone carotid endarterectomy were defined as having cerebrovascular disease (CVD). Patients with a history of claudication, ulceration or amputation due to ischemia of the limbs or those who underwent peripheral revascularization were defined with peripheral arterial disease (PAD). When the medical records documented presence of signs and symptoms compatible with heart failure, and/or echocardiography findings support to heart failure, patients were diagnosed with heart failure. Those patients taking antiarrhythmic medications, or electrocardiogram changes of rhythm and rate, were defined as having cardiac arrhythmia.

Biochemical Parameters

Routine laboratory investigations of all patients were performed every month before hemodialysis session and recorded monthly. Averages of the biochemical parameters of the last year were used for analysis. Serum levels of hemoglobin, iron, iron binding capacity, ferritin, total cholesterol, triglycerides, LDL cholesterol, HDL cholesterol, creatinine, glucose, sodium, potassium, uric acid, calcium, phosphorus, parathyroid hormone, calcium phosphorous product, albumin, CRP, glycated hemoglobin (HbA1c), single pool Kt/V, and urea reduction ratio (URR) were determined using routine laboratory techniques.

Follow-up and Outcomes

At the end of 2 years follow-up period, the cases of death, kidney transplant, and transfer to peritoneal dialysis were recorded. Causes of death were determined as cardivascular events, infections, and gastrointestinal bleeding. Based on survival status, the patients were divided into two groups: group I (the survival group) and group II (the death group). Patients who were received a kidney transplant, or for any reason transferred to peritoneal dialysis were excluded in the survival group. Patient characteristics and the risk factors of mortality were compared among two groups.

Statistical Analysis

Data were analyzed with Statistical Package of Social Science (SPSS Inc., Chicago, IL, USA) software version 11.5 for Windows. Tests for normality of distribution for continuous variables were performed using the Kolmogorov-Smirnov test. Data were expressed as mean ± standard deviation or median (interquartile range) for continuous variables according to their distribution and as number or percentages for categorical variables. The difference between the two groups was determined by independent t-test for numerical variables with normal distribution and by Mann-Whitney U test for numerical variables without normal distribution. Relationships between categorical variables were also determined by Pearson chi-square and Fischer’s exact test. Relationships between numerical variables were examined with Pearson’s correlation coefficient. Univariate logistic regression analysis was performed for all possible risk factors. In univariate analysis, variables with p<0.25 were accepted as candidate variables along with all known variables of clinical significance for the multivariate model. Variable selection for multiple models was made by Backward likelihood ratio method. Odds ratio and 95% confidence interval were calculated for each independent variable. The value of p<0.05 was considered statistically significant.

Results

Patients Characteristics

A total of 74 patients (53% females and 47% males) were included to analysis. All patients were under hemodialysis therapy three times peer week for 3-5 hours and the median dialysis vintage was 49 (3-324) months. The mean age of the study population was 53.04±14.61 years. Seventeen percent of the patients were classified as underweight, while 19% overweight and 11% obese. The etiologies of primary kidney disease were as follow: 19 (25.7) had diabetic kidney disease, four (5.4) had hypertensive kidney disease, eight (10.8) had glomerulonephritis, two (2.7) had polycystic kidney disease, two (2.7) had secondary amyloidosis, three (4.1) had reflux nephropathy, seven (9.5) had obstructive uropathy/nephrolithiasis, and 29 (39.2) had uncertain etiology. The majority of the patients (82.4%) had HT. Additionally, 28.4% had DM, 45.9% dyslipidemia, 25.7% CAD, 9.5% CVD, 6.8% PAD, 5.4% cardiac arrhythmia, and 2.7% heart failure. The prevalence of arteriovenous fistula usage was 83.8% in MHD patients. Eighty-eight percent of total patients were received erythropoiesis stimulating agents (ESA) and half of the patients were using darbepoetin The mean hemoglobin level of was 10.64±0.74 g/dL. The prevalence of mortality was 28.4% within 2 years, while annual rate of mortality was approximately 15%. The baseline characteristics of the study patients are presented in Table 1 and Table 2.

Follow-up and Comparison of Two Groups

At the end of 2 years follow-up period, 21 (28.4) patients had died, and four (5.4) had received a kidney transplant. The leading cause of death was cardiovascular events (52.2%), followed by infections (43%), and gastrointestinal bleeding (4.8%). The mean age of the patients that died during the study period were significantly higher than that the survival patients (59.66±11.89 vs 51.71±14.48; p=0.030). Compared with the survival group, the death group patients had higher rate of comorbid conditions such as DM, HT, CAD, CVD, and PAD. However, statistically significant differences were found for HT and CVD between two goups (p=0.027 vs p=0.022, respectively). There were no statistically significant differences between two groups in most clinical parameters such as gender, BMI, etiology of primary kidney disease, ultrafiltration volume, type of vascular access, viral serology, ESA usage, and smoking status. The median hemoglobin levels were not significantly different among the groups (10.4 g/dL vs 10.6 g/dL; p=0.095). In contrast, in the death group patients required significantly higher ESA doses than in the survival groups (0.56±0.13 µg/kg/week vs 0.46±0.15 µg/kg/week; p=0.014). The death group had lower median dialysis vintage than that the survival group, but not reaching statistically significance (32 months vs 54 months, p=0.057). The death group patients had significantly lower levels of serum creatinine (p=0.005) and albumin (p=0.012), but higher levels of CRP (p=0.026). The characteristics and comparisons of two groups are presented in Table 3.

To identify potential risk factors for mortality, we conducted logistic regression analysis. On univariate analysis, age (≥65 years), presence of CVD, levels of serum albumin lower than 3.5 g/dL, and elevated CRP levels were found associated with mortality. On multivariate analysis, the presence of DM [odds ratio (OR)=3.583, 95% confidence interval (CI): 1.003-12.808, p=0.05], CVD (OR=8.217, 95% CI: 1.263-53.442, p=0.027), and elevated CRP levels (OR=4.340, 95% CI: 1.306-14.416, p=0.017) were independent risk factors for mortality. Factors associated with mortality are presented in Table 4.

Discussion

In this study, we retrospectively examined 74 patients on MHD in order to identify parameters that could be associated with mortality. During the follow-up period, 21 patients died and four patients received a kidney transplant. The annual rate of mortality was 15%. The causes of death were cardiovascular events for 11 patients, infections for nine, and gastrointestinal bleeding for one. Patients who died were older, had DM, HT, CAD, CVD and PAD, and required higher ESA doses to achieve the target hemoglobin levels. In addition, the death group patients had lower levels of serum creatinine and albumin, while had higher levels of CRP. The presence of DM, CVD, and elevated CRP levels seem to be independent risk factors for mortality in MHD patients.

In spite of the considerable advances in the public health, MHD patients have high mortality rates. Almost half of the patients die due to cardiovascular disease. The high risk of cardiovascular mortality is associated with a high prevalence of known traditional cardiovascular risk factors. In addition, many MHD patients are of older age and have multiple comorbid conditions, as well as dysfunction of the immune system (3). Non-cardiovascular mortality is also increased on MHD patients, but the importance of non-cardiovascular mortality is often overlooked (19). de Jager et al. (4) analyzed data from the European Renal Association–European Dialysis and Transplant Association Registry and showed that in dialysis patients mortality from non-cardiovascular disease was increased to the same extent as mortality from cardiovascular disease. Shastri et al. (20) reported the various causes of death in a large cohort of patients undergoing hemodialysis and showed that rates of sudden cardiac death, non-sudden cardiac death, and non-cardiac death were 22%, 17%, and 61%, respectively. According to the Turkish Society of Nephrology’s 2018 Registry Report, the most common cause of death in prevalent hemodialysis patients was cardiovascular (48%), followed by cerebrovascular (14%), malignancy (10.4%), and infection (10%) (21). In our study, during the follow-up period, 21 (28.4) patients died and the rates of death for cardiovascular events, infections, and gastrointestinal bleeding were 52.2%, 43%, and 4.8%, respectively. In our study, cardiovascular and cerebrovascular deaths were evaluated together as cardiovascular events. Cardiovascular deaths were the first among the causes of death. These findings were in accordance with the literature.

Inflammation plays an important role in the mortality in patients on chronic hemodialysis. CRP as a marker of inflammation can be used as a predictors of death. In a study on 280 hemodialysis patients, CRP was found to be strongly independent predictors of both overall death and cardiovascular death (22). This was in agreement with our study results. The relation between the type of vascular access and mortality has been reported in hemodialysis patients (23). The presence of a non-native vascular access device, including a CVC is the most common identifiable source of infection in patients on hemodialysis that contributes to increased mortality (24). In our study cohort, we found that the patients who died, as compared to living patients had a higher percentage of them used CVC for vascular access. However, it was not reaching statistically significance. This may be related to relatively small sample size.

The median hemoglobin level of our patients was 10.6 g/dL. Compared with the survival group, the death group had a lower hemoglobin levels and they required significantly higher ESA doses. Limited evidence suggests that an increased mortality may be due to high ESA doses. In one observational study of hemodialysis patients in the United States, in patients with persistently low hemoglobin levels (<9.5 g/dL) and received larger ESA dose changes over an 11-month period had a higher mortality risk (25). In another study, lower erythropoietin responsiveness was a strong, independent predictor of mortality risk (26). However the association between higher ESA doses and mortality has not been shown in all studies. In one observational study, on average, epoetin dosages >30,000 unit/week were not confer additional harm or benefit in elderly hemodialysis patients (27).

Previous studies demonstrated that older age, previous atherosclerotic vascular disease, DM, higher levels of CRP and lower levels of albumin are associated with mortality in patients undergoing hemodialysis (28). Our results were in accordance with these studies. We found that most of hemodialysis patients were died secondary to atherosclerotic cardiovascular disease. Infection was the second leading cause of mortality in these patients. The death group patients were older, had higher comorbid conditions such as DM, HT, CAD, CVD and PAD, lower serum albumin and creatinine levels, and higher CRP levels when compared with the survival group. In univariate analysis, patients 65 years of age and older and serum albumin levels below 3.5 g/dL were found associated with mortality, but in multivariate analysis did not show. We also found that DM, CVD, and higher CRP levels are three main predictors of mortality in MHD patients.

The assessment of nutritional status among hemodialysis patients is very important because of as a predictor of morbidity and mortality (29). However, there is no gold standart parameters in routine clinical practice for nutritional status assessment. Different markers such as serum albumin, creatinine, BMI, and lipid profile have been associated with mortality (30). Malnutrition associated with inflammation has been extensively studied in recent years and is considered a risk factor that increase the mortality in hemodialysis patients (31,32). We evaluated the nutritional status by BMI, pre-dialysis serum albumin, creatinine, and lipid profile of patients. Although total cholesterol, triglycerides, and BMI were lower in the non-survival patients, there were not statistically significant among the groups. Only serum creatinine levels were lower in the died patients. With regard to hemodialysis efficacy, total Kt/V, ultrafiltration volume, and URR did not effect mortality in the present study. Regarding the mineral metabolism disorders serum calchium, phosphorus, parathyroid hormone, and calchium phosphorus product were not differ in both group of patients.

Study Limitations

Our study has some limitations. First, the number of patients included in this study and follow-up period were relatively limited. Second, as the baseline data were recorded at the start of the study, events occurring 2 years follow-up period may be explained by the course of the patients and not by the baseline status.

Conclusion

A careful evaluation are essential in MHD patients especially with DM, CVD, and high CRP levels. This provides to achieve timely the clinical goals and to improve survival in this group of patients.